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Test Code PM3CX Lymph3Cx Assay, Primary Mediastinal Large B-cell Lymphoma and Diffuse Large B-cell Lymphoma, mRNA Gene Expression, NanoString, Tissue


Ordering Guidance


This test does not include a pathology consultation. If a pathology consultation is requested, PATHC / Pathology Consultation will be ordered and performed at an additional charge.



Necessary Information


Pathology report (final or preliminary) must accompany specimen in order for testing to be performed. At minimum, it should contain the following information:

1. Patient name

2. Block number-must be on all blocks, slides, and paperwork (can be handwritten on the paperwork)

3. Tissue collection date

4. Source of the tissue



Specimen Required


Submit only 1 of the following specimens:

Preferred:

Specimen Type: Tissue slides

Slides: 1 Hematoxylin and eosin stained and 6 to 10 unstained

Collection Instructions:

1. Submit 1 slide stained with hematoxylin and eosin.

2. If the formalin-fixed paraffin-embedded (FFPE) biopsy specimen is a core needle biopsy or fine needle aspirate, submit 10 unstained slides.

3. If the FFPE biopsy specimen is a resection, submit 6 unstained slides.

4. Submitted slides should be 4- to 5-micron thick sections placed on positively charged slides.

Additional Information: Paraffin embedded specimens can be from any anatomic location (skin, soft tissue, lymph node, etc).

 

Acceptable:

Specimen Type: Tissue block

Collection Instructions: Submit an FFPE tumor tissue block.

Specimen Minimum Volume

Minimum 60% tumor with or without macrodissection. Minimum required unstained tissue section input: 0.12 mm(3); Slides: See Specimen Required


Useful For

Only indicated for formalin-fixed paraffin-embedded specimens from patients diagnosed with large B-cell lymphoma

Additional Tests

Test ID Reporting Name Available Separately Always Performed
AZSLR Slide Review No, (Bill Only) Yes

Testing Algorithm

When this test is ordered, slide review will always be performed at an additional charge.

Method Name

Gene Expression by Digital Counting of Fluorescent Bar-Coded Probes

Reporting Name

Lymph3Cx, Lg Bcell Lymphoma,mRNA,Ts

Specimen Type

Tissue, Paraffin

Specimen Minimum Volume

Minimum 60% tumor with or without macrodissection. Minimum required unstained tissue section input: 0.12 mm(3); Slides: See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time
Tissue, Paraffin Ambient (preferred)
  Refrigerated 

Reject Due To

Exhausted tissue block Reject
Tissue with non-formalin fixation Reject
  All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Reference Values

Not applicable

Day(s) Performed

Monday through Friday

Report Available

7 to 12 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

0120U

88381

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PM3CX Lymph3Cx, Lg Bcell Lymphoma,mRNA,Ts 93785-4

 

Result ID Test Result Name Result LOINC Value
606164 Lymph3Cx, Large B-cell Lymphoma, mRNA, Tissue 93779-7
606165 PMBCL Probability 93780-5
606185 PMBCL Call 93782-1
606166 DLBCL Probability 93781-3
606186 DLBCL COO 93783-9