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HelpTest Code FGEN Fungal Culture, Routine
Reporting Name
Fungal Culture, RoutineUseful For
Diagnosing fungal infections from specimens other than blood, skin, hair, nails, and vagina (separate tests are available for these specimen sites)
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
VariesOrdering Guidance
Nocardia and the other aerobic actinomycetes are not fungi, therefore a fungal culture should not be ordered. However, these organisms grow well on mycobacterial medium. When infection with this group of organisms is suspected, order CTB / Mycobacteria and Nocardia Culture, Varies.
Shipping Instructions
Specimen should arrive within 24 hours of collection.
Necessary Information
Specimen source is required.
Specimen Required
Submit only 1 of the following specimens:
Preferred:
Specimen Type: Body fluid
Container/Tube: Sterile container
Specimen Volume: Entire collection
Specimen Type: Fresh tissue
Container/Tube: Sterile container
Specimen Volume: Pea size
Collection Instructions: Tissue should be placed in small amount of sterile saline or sterile water.
Acceptable:
Specimen Type: Bone marrow
Container/Tube: Sterile container, SPS/Isolator system, or green top (lithium or sodium heparin)
Specimen Volume: Entire collection
Specimen Type: Respiratory specimen
Container/Tube: Sterile container
Specimen Volume: Entire collection
Specimen Type: Urine
Container/Tube: Sterile container
Specimen Volume: 2 mL
Collection Instructions: Collect a random urine specimen.
Fresh tissue or body fluid are preferred over a swab specimen. Recovery of mycobacteria from swabs is generally very low yield. Only submit a swab specimen if tissue biopsy or fluid aspiration is not possible.
Specimen Type: Swab
Supplies: BD E-Swab (T853)
Sources: Dermal, ear, mouth, ocular, throat, or wound
Container/Tube: Sterile, screw-capped tube containing Liquid Amies Medium with flocked swab (eg, E-Swab)
Specimen Volume: 1 mL in swab container with swab
Collection Instructions:
1. Before collecting specimen, wipe away any excessive amount of secretion and discharge, if appropriate.
2. Obtain secretions or fluid from source with sterile flocked swab. Paranasal sinus collections must use a nasopharyngeal flocked swab.
3. Place flocked swab in sterile, screw-capped tube containing 1 mL of Liquid Amies Medium.
4. If smear and culture are requested or both a bacterial culture and fungal culture are requested, collect a second swab to maximize test sensitivity. Submit each swab in a separate sterile, screw-capped tube with 1 mL of Liquid Amies Medium
Specimen Minimum Volume
Bone marrow or body fluid: 1 mL; Cerebrospinal fluid: 0.5 mL; Respiratory specimen: 1.5 mL; All other specimen types: See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Refrigerated (preferred) | 7 days |
| Ambient | 7 days |
Reference Values
Negative
If positive, fungus will be identified.
Day(s) Performed
Monday through Sunday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
87102-Fungal culture, routine
87106-Id MALDI-TOF Mass Spec Yeast (if appropriate)
87107-Id MALDI-TOF Mass Spec Fungi (if appropriate)
87107-Fungal identification panel A (if appropriate)
87107-Fungal identification panel B (if appropriate)
87150 x 2-Identification Histoplasma/Blastomyces, PCR (if appropriate)
87153-D2 fungal sequencing identification (if appropriate)
87176-Tissue processing (if appropriate)
87150- Id, Candida auris Rapid PCR (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| FGEN | Fungal Culture, Routine | 51723-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| FGEN | Fungal Culture, Routine | 51723-5 |
Report Available
24 to 35 daysReject Due To
| Blood or fixed tissue Specimen in viral transport medium (including but not limited to M4, M5, BD viral transport media, thioglycolate broth) Swab sources of respiratory fluids (eg, sputum) or nasal swab Wood shaft or charcoal swab Catheter tips Petri dish Stool Blades from scalpels or razors Boric acid tubes Aptima swab Culture transport swabs (eg, Culturette) |
Reject |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| D2F | D2 Fungal Sequencing Identification | No, (Bill Only) | No |
| FUNA | Fungal Ident Panel A | No, (Bill Only) | No |
| FUNB | Fungal Ident Panel B | No, (Bill Only) | No |
| LCCI | Ident Rapid PCR Coccidioides | No, (Bill Only) | No |
| LCHB | Id, Histoplasma/Blastomyces PCR | No, (Bill Only) | No |
| RMALF | Id MALDI-TOF Mass Spec Fungi | No, (Bill Only) | No |
| TISSR | Tissue Processing | No, (Bill Only) | No |
| RMALY | Id MALDI-TOF Mass Spec Yeast | No, (Bill Only) | No |
| LCCA | Id, Candida auris Rapid PCR | No, (Bill Only) | No |
Testing Algorithm
When this test is ordered, the reflex tests may be performed at an additional charge.
For more information see Meningitis/Encephalitis Panel Algorithm.
Method Name
Conventional Agar Culture/Macroscopy/Microscopy/D2 rDNA Gene Sequencing/Real-Time Polymerase Chain Reaction (rtPCR)/Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS)
Dimorphic Pathogen Identification Confirmation: D2 rDNA Gene Sequencing/rtPCR/MALDI-TOF MS
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.