Test Code AH50 Alternative Complement Pathway, Functional, Serum
Reporting Name
Alternative Complement Path Func, SUseful For
Investigation of suspected alternative pathway complement deficiency, atypical hemolytic uremic syndrome, C3 glomerulonephritis, and dense-deposit disease
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Performing Laboratory

Specimen Type
SerumOrdering Guidance
COM / Complement, Total, Serum and this test are the most appropriate primary assays to use as screening methods for complement deficiencies. Abnormal results in one or the other, neither or both assays will help direct further testing.
This test is rarely useful when ordered in isolation.
Specimen Required
Patient Preparation: Fasting preferred
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Immediately after specimen collection, place the tube on wet ice and allow specimen to clot.
2. Centrifuge at 4° C and aliquot serum into a plastic vial.
3. Freeze specimen within 30 minutes of centrifugation. Sample must be placed on dry ice if not frozen immediately.
NOTE: If a refrigerated centrifuge is not available, it is acceptable to use a room temperature centrifuge, provided the specimen is kept on ice before centrifugation, and immediately afterward, the serum aliquoted and frozen.
Specimen Minimum Volume
0.75 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Frozen | 14 days |
Reject Due To
Gross hemolysis | OK |
Gross lipemia | OK |
Gross icterus | OK |
Reference Values
≥46% normal
Day(s) Performed
Varies
CPT Code Information
86161
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
AH50 | Alternative Complement Path Func, S | 74520-8 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
88676 | Alternative Complement Path Func, S | 74520-8 |
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.Report Available
3 to 5 daysForms
If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.